Alkaloid secondary metabolites from a plant endophytic fungus Purpureocillium lilacinum
An endophytic fungus strain GZY-Z-02 was isolated from the leaves of Paraboea sinensis and identified as Purpureocillium lilacinum by molecular biological method.The fungus was fermented on rice medium for 30 days at room temperature,and the crude extracts were obtained by extraction with ester acetate.By means of silica gel column chromatography,Sephadex LH-20 and HPLC twelve metabolites were obtained.The twelve metabolites were identified through NMR,MS and CD spectra,which all belong to alkaloids,including a new compound lilacinumopin(1),five diketopiperazines(2-6),two indole analogs(7-8),three nucleoside derivatives(9-11)and Vitamin B2(12).An antimicrobial activity assay proved that the compounds 1,7,and 8 displayed selectively moderate antibacterial activity to Staphylococcus aureus.
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