Optimization of liquid fermentation medium for Auricularia sinodelicata strain based on response surface methodology
Using single-factor test and response surface design method,the biofermentation processes,including formulation of the liquid fermentation medium and incubation time of the wild strain of Auricularia sinodelicata were optimized.The results indicated that the optimal shake culture temperature for the strain was 28 ℃,the rotation speed was 230 r/min,and the pH value of the medium was 6.0.The optimal carbon and nitrogen sources,and inorganic salts,were formulated as 30 g/L of fructose,2 g/L of yeast extract,and 2.5 g/L of potassium chloride,respectively.Using mycelial biomass as the response value,the optimal fermentation medium formulation based on the Box-Benhnken design was predicted to be 38.424 g/L of fructose as carbon source,4 g/L of yeast extract as nitrogen source,and 2.736 g/L of potassium chloride.Under the same culture conditions,the measured dry weight of mycelial biomass in 7 d of fermentation was 2.163 6 g/100 mL or 166.18％of the theoretical value,indicating the feasibility of the study and the reliability of the conclusion.Maximum mycelial biomass was(2.530 0± 0.290 0)g/100 mL and the cellulase activity was 82.586 6 U/mL in the optimal incubation time of 8 d.This study aims at providing a reference for selecting high-yielding strains in artificial liquid cultivation of A.sinodelicata.
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