基于响应面法对中国皱木耳菌种液体发酵培养基优化
Optimization of liquid fermentation medium for Auricularia sinodelicata strain based on response surface methodology
许娟 1徐彦军 1江苏燕 1李伟 1查兴雪1
作者信息
- 1. 贵州大学农学院/贵州大学食用菌研究院 贵州省高等学校设施蔬菜工程研究中心,贵州贵阳 550025
- 折叠
摘要
以野生中国皱木耳菌株为试材,采用单因素试验及响应面法对其液体发酵培养基配方及培养时间等生物发酵过程进行优化,结果表明中国皱木耳液体菌种摇床培养适宜温度为28 ℃、转数为230r/min、培养基pH值为6,最佳碳氮源、无机盐及其添加量分别为果糖30 g/L、酵母浸膏2g/L、氯化钾2.5 g/L;进一步以菌丝生物量为响应值,依据Box-Benhnken设计预测最适发酵培养基配方为碳源果糖38.424 g/L、氮源酵母浸膏4g/L、氯化钾2.736 g/L.在相同培养条件下,7d时菌丝生物量干重实测值为2.163 6g/100mL,且为理论值的166.18％,说明本研究方法可行、结论可靠.通过测定纤维素酶活性及菌丝生物量,得到最佳培养时间为8 d,此时菌丝生物量最高可达(2.530 0±0.290 0)g/100 mL,纤维素酶活为82.586 6 U/mL.本研究优化了中国皱木耳液体菌种发酵生产条件,以期为中国皱木耳人工栽培时的液体菌种生产提供参考依据.
Abstract
Using single-factor test and response surface design method,the biofermentation processes,including formulation of the liquid fermentation medium and incubation time of the wild strain of Auricularia sinodelicata were optimized.The results indicated that the optimal shake culture temperature for the strain was 28 ℃,the rotation speed was 230 r/min,and the pH value of the medium was 6.0.The optimal carbon and nitrogen sources,and inorganic salts,were formulated as 30 g/L of fructose,2 g/L of yeast extract,and 2.5 g/L of potassium chloride,respectively.Using mycelial biomass as the response value,the optimal fermentation medium formulation based on the Box-Benhnken design was predicted to be 38.424 g/L of fructose as carbon source,4 g/L of yeast extract as nitrogen source,and 2.736 g/L of potassium chloride.Under the same culture conditions,the measured dry weight of mycelial biomass in 7 d of fermentation was 2.163 6 g/100 mL or 166.18％of the theoretical value,indicating the feasibility of the study and the reliability of the conclusion.Maximum mycelial biomass was(2.530 0± 0.290 0)g/100 mL and the cellulase activity was 82.586 6 U/mL in the optimal incubation time of 8 d.This study aims at providing a reference for selecting high-yielding strains in artificial liquid cultivation of A.sinodelicata.
关键词
菌丝生物量/液体发酵/纤维素酶活性/响应面法Key words
mycelial biomass/liquid fermentation/cellulase activity/response surface methodology引用本文复制引用
基金项目
贵州省高等学校设施蔬菜工程研究中心平台建设项目(黔教技[2022]040)
贵州省科技重大专项(黔科合重大专项[2019]3005)
出版年
2024
国家科技期刊平台
NSTL
万方数据