Mechanisms of proliferation,migration and apoptosis of 786-O and ACHN renal carcinoma cell lines stimulated by cordycepin
The mechanism of apoptosis and migration of renal cancer cell lines after stimulation by cordycepin administration was explored.Kidney cancer 786-O cell line and kidney cancer ACHN cell line were cultured in vitro,and MTT method,cell migration experiment,HE staining,immunofluorescence staining,and protein immunoimprinting were used to examine the inhibition rate of proliferation,cell migration,cell morphology and nuclear morphology of renal cancer cell lines under different concentration of cordycepin administration.The expression of apoptosis-related gene proteins was investigated,and the mechanism of apoptosis induced by cordycepin in renal cancer cells was explored.With the increase of concentration and dose,cordycepin can significantly promote apoptosis and inhibit migration of renal cancer cell lines.Morphological and HE staining observation showed that the number of cancer cells was significantly reduced,and the nucleus was significantly enlarged.Immunofluorescence staining showed that the expression of MMP-2,MMP-9 and BCL-2 proteins decreased significantly,the expression of Bax,Casepase-3 and Casepase-7 proteins increased significantly,and the apoptosis renal cancer cells could be induced through the AKT/mTOR signaling pathway.
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