摘要
以SD大鼠为研究对象,用霍恩氏法(Horn法)计算亚稀褶红菇的半数致死剂量(LD50),测定亚稀褶红菇对大鼠尿素氮(BUN)、肌酐(CREA)、钾(K)、钠(Na)、氯(Cl)、白细胞(WBC)计数及分类、尿蛋白、尿糖和潜血水平的影响,并对左侧肾脏进行病理学观察.亚稀褶红菇对雌性SD大鼠的 LD50及95％可信区间(95％CI)为 271(169-441)mg/kg,雄性 SD 大鼠的 LD50及95％CI为 233(143-378)mg/kg;与对照组比,各亚稀褶红菇组SD大鼠BUN、CREA、K、WBC计数、中性粒细胞比例、尿蛋白、尿糖和潜血的发生率及严重程度均增加;各亚稀褶红菇组SD大鼠Na和Cl均降低;各亚稀褶红菇组大鼠SD肾小管坏死,表现为上皮细胞空泡变性和水样变性,刷状缘消失,管腔内可见脱落的上皮细胞;且随着亚稀褶红菇剂量的增加,各指标变化更显著.亚稀褶红菇可导致SD大鼠肾损伤,并呈剂量反应关系.
Abstract
SD rats were dosed with Russula subnigricans,and Horn method was used to calculate the median lethal dose(LD50).The effects of R.subnigricans on urea nitrogen(BUN),creatinine(CREA),potassium(K),sodium(Na),chlorine(Cl),white blood cell(WBC)count and classification,urinary protein,urine sugar and occult blood levels in SD rats were determined,and pathology of left kidney was observed.The LD50 and 95％confidence interval(95％CI)of female SD rats were 271(169-441)mg/kg,and those of male SD rats were 233(143-378)mg/kg.Compared with the control,the BUN,CREA,K,WBC counts,neutrophil ratio,the incidences and severity of urine protein,urine sugar and occult blood of SD rats dosed with R.subnigricans were increased,while Na and Cl were decreased.The renal tubular necrosis of SD rats in each treatment groups showed vacuolar degeneration,watery degeneration,and the brush margin disappearance of the epithelial cells,and exfoliated epithelial cells were visible in the lumen.With the increase of the dosage of R.subnigricans,the change of each index was more significant.The R.subnigricans could lead to kidney injury in rats in a dose-response relationship.
基金项目
贵州省科技厅课题(黔科合基础[2017]1095)
国家科技期刊平台
NSTL
万方数据