Effects of Russula subnigricans on renal function in SD rats
SD rats were dosed with Russula subnigricans,and Horn method was used to calculate the median lethal dose(LD50).The effects of R.subnigricans on urea nitrogen(BUN),creatinine(CREA),potassium(K),sodium(Na),chlorine(Cl),white blood cell(WBC)count and classification,urinary protein,urine sugar and occult blood levels in SD rats were determined,and pathology of left kidney was observed.The LD50 and 95％confidence interval(95％CI)of female SD rats were 271(169-441)mg/kg,and those of male SD rats were 233(143-378)mg/kg.Compared with the control,the BUN,CREA,K,WBC counts,neutrophil ratio,the incidences and severity of urine protein,urine sugar and occult blood of SD rats dosed with R.subnigricans were increased,while Na and Cl were decreased.The renal tubular necrosis of SD rats in each treatment groups showed vacuolar degeneration,watery degeneration,and the brush margin disappearance of the epithelial cells,and exfoliated epithelial cells were visible in the lumen.With the increase of the dosage of R.subnigricans,the change of each index was more significant.The R.subnigricans could lead to kidney injury in rats in a dose-response relationship.
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