摘要
从贵州黔南布依族苗族自治州和黔西六盘水市农家自制腊肉表面分离得到的霉菌经形态学和基于钙调蛋白基因(calmodulin gene,CaM)、RNA多聚酶Ⅱ第二大亚基基因(DNA-dependent RNA polymerase Ⅱsecond largest subunit gene,Rpb2)和 rDNA ITS1-5.8S-ITS2(ITS)序列的分析,鉴定出曲霉Aspergillus 8种和青霉Penicillium 1种,即堆积曲霉A.cumulatus、黄曲霉A.flavus、灰绿曲霉A.glaucus、詹森曲霉A.jensenii、多育曲霉A.proliferans、伪灰绿曲霉A.pseudoglaucus、田纳西曲霉A.tennesseensis、产毒曲霉 A.venenatus 和橘紫青霉 P.aurantioviolaceum.其中 A.cumulatus、A.venenatus 和 P.aurantioviolaceum 为中国 3 个新记录种.
Abstract
Based on morphological taxonomic examination and molecular phylogenetic analysis of partial calmodulin gene(CaM),DNA-dependent RNA polymerase Ⅱ second largest subunit gene(Rpb2)and rDNA ITS1-5.8S-ITS2(ITS)sequences,the moulds isolated from surface of the home-made dry-cured meat in Buyi and Miao Autonomous Prefecture and Liupanshui City in Guizhou Province are identified as eight Aspergillus species and one Penicillium species,namely,A.cumulatus,A.flavus,A.glaucus,A.jensenii,A.proliferans,A.pseudoglaucus,A.tennesseensis,A.venenatus and P.aurantioviolaceum;among which,A.cumulatus,A.venenatus and P.aurantioviolaceum are new to China.
基金项目
国家自然科学基金(U20A20101)
科技部基础资源调查专项(2019FY100700)
国家科技期刊平台
NSTL
万方数据