Geosmithia armandii,a new fungal species associated with Tomicus armandii and its pathogenicity to Pinus armandii
Species of Geosmithia(Ascomycota,Hypocreales)are commonly associated with bark beetles,and several species can infect tree hosts and cause serious diseases.In this study,a culture-dependent and pathogenic trial was conducted to classify a species of Geosmithia associated with Tomicus armandii infesting Pinus armandii in southwest China.In total,387 Geosmithia isolates were obtained from 45 adults and 64 galleries of T.armandii in Yuxi and Fuyuan Cities,Yunnan Province,China.Ten representative isolates were further identified on the basis of morphological and physiological characteristics and the phylogenetic analyses were conducted based on ITS,TUB2,and TEF1-α sequences.Among these,90 isolates were used for the establishment of the ITS tree,66 of the TUB tree,and 77 of the TEF tree.As a result,a novel species,G.armandii was identified.This is the first species of Geosmithia reported to be associated with T.armandii.In vivo pathogenicity analysis of the two isolates selected indicated that in one month after inoculation,there was no significant difference between average lesion length caused by the inoculated isolates and the experimental control,showing that G.armandii is a weak pathogen of Pinus armandii.
Geosmithiamorphologyphylogenetic analysispathogenicitynew species
万方数据
维普
