Transcriptome was used to preliminarily explore the mechanism of trichostatin A delaying the formation of brown mycelium membrane of Lentinula edodes
Histone deacetylation is one of the most important epigenetic modifications and plays a variety of roles in development,but the detailed function and mechanism of histone deacetylation in Lentinula edodes is unknown.An extensive deacetylation inhibitor(HDACi),trichostatin A(TSA)was used to determine the role of histone deacetylation on the developmental process of Lentinula edodes.It was observed that exogenous application of TSA could inhibit the process of ripening and brown mycelium film formation of pack-cultured Lentinula edodes.Transcriptomic analysis revealed that TSA treatment produced 393 differentially expressed genes,which were mainly enriched in the biosynthesis of secondary metabolites,protein processing in the endoplasmic reticulum,and microbial metabolism in different environments.Analysis of the differences in gene expression between TSA treatment and control groups indicated that TSA might directly or indirectly affect the genes of cytochrome P450,laccase,and glycoprotein family by affecting histone acetylation levels,and thereby exert an influence on the formation of Lentinula edodes brown mycelium membrane.This paper provides a reference helpful to shortening the cycle of industrial production of Lentinula edodes.
transcriptomehistone deacetylationLentinula edodes brown mycelium film formationtrichostatin A
万方数据
维普
