Candidate transcription factor regulating the production of sanxiapeptin by Penicillium oxalicum
Penicillium oxalicum is an important biocontrol fungus.Previously,a new linear pentapeptide,sanxiapeptin,was obtained from the fermentation product of P.oxalicum isolated from vicinity of citrus orchard,which hada potent inhibitory effect on rot-causal fungus P.digitatum on citrus.To reveal the molecular regulatory mechanism and increase the production of sanxiapeptin,the transcriptome data of different strains of P.oxalicum previously determined under various cultural conditions were systematically analyzed.By establishing sets of differentially expressed genes among different comparison groups and taking the intersection of each set,nine candidate transcription factors were screened from 478 transcription factors that were highly correlated with the production level of sanxiapeptin,of which three were negatively and six were positively correlated with sanxiapeptin production.Bioinformatics analysis of the candidate transcription factors and their coding genes was performed,and the accuracy of transcriptome data was verified through qRT-PCR.This study provides a theoretical foundation for analyzing the molecular regulatory mechanism of sanxiapeptin and promoting the development of fruit and vegetable preservatives using sanxiapeptin as main component.It also serves as a reference for mining target genes through transcriptome data.
万方数据
维普
