Inhibitory effects of mycoviruse BmPV1 on Bipolaris maydis infection
Corn southern leaf blight is one of the important diseases in maize production in China,causing significant yield reduction every year.Bipolaris maydis partitivirus 1(BmPV1)is a dsRNA virus carried by B.maydis,the pathogens of corn southern leaf blight.It has hypovirulence and can reduce the pathogenicity of B.maydis,but the effect of BmPV1 on host infection is not clear.In this study,the Agrobacterium-mediated transformation method was used to introduce the gfp gene into the BmPV1 carrying strain 3086-BmPV1 and the non-virus strain 3086-non of B.maydis.The stability of the fluorescent-labeled strains was analyzed by PCR and fluorescence observation.The effect of the introduction of gfp gene on biological characteristics of the fluorescent-labeled strains were analyzed.Spore suspensions were sprayed on maize leaves for inoculation,and the infection of B.maydis on maize leaves was observed using fluorescence microscopy.PCR amplification and fluorescence observation results showed that the gfp gene was successfully integrated into strains 3086-BmPV1 and 3086-non.There were no significant differences in growth rate,sporulation,and pathogenicity between the fluorescent-labeled strains and the non-labeled strains.The infection results revealed that the non-virus strain 3086-non-GFP could invade maize leaves through two pathways:directly infecting epidermal cells and spreading to adjacent cells,or invading stomata and spreading through intercellular spaces.The virus-carrying strain 3086-BmPV1-GFP could only grow on the leaf surface and had minimal ability to infect epidermal cells.This study clarifies that the BmPV1 virus can reduce the pathogenicity and inhibit the infection of B.maydis.
corn southern leaf blightmycoviruse BmPV1hypovirulencefluorescence labelinginfection process
万方数据
维普
