首页|优化蒸汽爆破条件辅助提取荷叶离褶伞多糖及其理化性质研究

优化蒸汽爆破条件辅助提取荷叶离褶伞多糖及其理化性质研究

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以荷叶离褶伞子实体为试验原料,采用蒸汽爆破辅助热水提取荷叶离褶伞多糖.综合考虑粗多糖得率、多糖含量及多糖提取率,在单因素试验基础上,采用响应面法优化蒸汽爆破处理工艺,得到最佳条件为压力 2.0 MPa,维压时间 90 s,物料大小 3 mm.在此条件下,荷叶离褶伞粗多糖得率为(20.09±0.20)%,是对照组(未蒸汽爆破处理)的 2.05 倍,多糖含量为(62.96±0.48)%,是对照组的 1.70 倍,多糖提取率为(12.65±0.15)%,是对照组的 3.49 倍.此外,对蒸汽爆破处理前后的多糖进行了理化性质对比,发现处理后β-葡聚糖含量是对照组的2.28倍.红外光谱表明蒸汽爆破处理后仍具有多糖典型吸收峰,但处理前后分子量分布特征差异较大,且经过蒸汽爆破处理所得多糖的单糖组成中葡萄糖占比增加,这与其β-葡聚糖含量升高相一致.免疫活性比较结果显示经过蒸汽爆破处理后富集β-葡聚糖的荷叶离褶伞多糖活性显著优于对照组荷叶离褶伞子实体多糖.
Optimization of steam explosion conditions for assisting extraction of Lyophyllum decastes polysaccharides and physicochemical properties of the polysaccharides extracted
Steam explosion was used to assist hot water extraction of Lyophyllum decastes polysaccharides.Considering the yield of the crude polysaccharide,total sugar content and polysaccharide extraction rate,the conditions of steam explosion process was optimized by using response surface method on the basis of one-way test.The optimum conditions obtained were 2.0 MPa of pressure,90 s of pressure maintenance and 3 mm of material size.Under optimized conditions,the yield of the crude polysaccharide,the total sugar content and the extraction rate were 2.05,1.70 and 3.49 times higher than that of the untreated control group.The content of β-glucan was 2.28 times higher than that of the control.The infrared spectra showed that the typical absorption peaks of polysaccharides still existed after the steam explosion treatment,but the molecular weight distribution characteristics differed greatly before and after the treatment,and the proportion of glucose in the monosaccharide composition of the polysaccharides obtained by the steam explosion treatment was increased,being consistent with the elevation of the β-glucan content.Cell culture test indicated that the immunomodulatory effect of polysaccharides obtained after steam explosion treatment were significantly improved as compared with that of polysaccharides of untreated control,possible due to the higher percentage of β-glucan in the treating samples.

steam explosionLyophyllum decastes polysaccharideresponse surface optimizationphysicochemical propertiesimmunological activity

罗羽、沈秀霖、张劲松、冯杰、刘利平、郭庆彬、刘艳芳

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天津科技大学食品科学与工程学院,天津 300457

上海市农业科学院食用菌研究所 农业农村部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点实验室,上海 201403

蒸汽爆破 荷叶离褶伞多糖 响应面优化 理化性质 免疫活性

上海市科技兴农重点攻关项目国家现代农业产业技术体系上海市农业科学院卓越团队建设计划

X2021-02-08-00-12-F00797CARS-20G2022003

2024

10.13346/j.mycosystema.230245

菌物学报
中科院微生物研究所 中国菌物学会

菌物学报

CSTPCD北大核心
影响因子：1.187
ISSN：1672-6472
年,卷(期)：2024.43(6)