杨树广泛应用于木材生产、防护林和园林景观建造,具有显著的经济价值和生态价值.杨树叶锈病主要为害杨树叶片,幼苗叶片更容易受害,2023 年河北邯郸杨树苗圃叶锈病发生严重危害.本研究针对河北邯郸及北京发生的杨树叶锈病病菌,通过观察夏孢子形态特征并结合 rDNA ITS 序列差异进行鉴定.结果发现,采自两地的杨树叶锈病菌均为松杨栅锈菌 Melampsora larici-populina,未发现我国对外检疫杨树叶锈病菌 M.medusae.本研究详细比较了杨树叶锈病菌的ITS序列差异,可以将M.medusae与Melampsora属中其他种区分开,为后续杨树叶锈病病原的鉴定和监测提供了基础数据.
Poplar leaf rust fungi from Hebei's Handan and Beijing and detection of quarantine species Melampsora medusae
Poplars are widely used in wood production,shelterbelt,and garden landscape construction,with significant economic and ecological value.Poplar leaf rust mainly occurs on poplar leaves with a severe impact on seedling growth.In 2023,leaf rust occurred seriously in the poplar nursery in Handan of Hebei Province.In this study,the pathogen of poplar leaf rust from Beijing and Hebei was identified by observing morphological characteristics of urediniospores on leaves and sequence comparison of rDNA ITS regions.The results showed that the pathogen responsible for poplar leaf rust in Beijing and Hebei was Melampsora larici-populina.The quarantine species M.medusae was not detected in these test samples.Eight distinct loci of the ITS sequence were used to differentiate M.medusae from the other closely related Melampsora species,providing some data for monitoring the quarantine species M.medusae.
万方数据
维普
