Quarantine identification of Seifertia azaleae in rhododendron imported from Belgium
A fungal strain 8033-2 isolated in bud of rhododendron imported from Belgium was identified by morphological characteristics,sequence analysis of internal transcribed spacer(ITS)region,translation elongation factor(EF-1α)and RNA polymerase Ⅱ second largest subunit(RPB)gene and pathogenicity test.The colony of isolate 8033-2 is white,initially hairy,with more aerial hyphae,and turned into dense,grey,olive green when well developed and mature.Conidia single-celled,smooth,nearly spherical or elliptical,gray brown,with a size of 8.04(6.32-11.49)×5.60(4.38-7.40)µm.The sequence similarities of ITS,EF-1α and RPB genes between isolate 8033-2 and the corresponding sequences of Seifertia azaleae in GenBank were 100%,100%and 99.90%,respectively.The phylogenetic tree based on ITS,EF-1α and RPB gene sequence showed that isolate 8033-2 clustered with S.azaleae.Artificial wound inoculation caused typical symptom of brown necrosis in healthy buds.The isolate 8033-2 was identified as Seifertia azaleae,which is the first interception of this quarantine pest at border ports of China.
万方数据
维普
