Detection of Albonectria rigidiuscula based on enzyme mediated duplex exponential amplification
Albonectria rigidiuscula is listed as Chinese quarantine pest,and has been intercepted at many ports in China,which may cause invasion risks.A precise enzyme mediated duplex exponential amplification(EmDEA)assay was developed for detection of A.rigidiuscula.Based on translation elongation factor(TEF1-α)gene of A.rigidiuscula and related species,five upstream DNA primers,six downstream DNA primers,and four RNA probes were designed and synthesized.The minimum detection limit is up to 0.5 pg/uL.The detection results of suspected sample indicate this method is useful to detect and screen A.rigidiuscula.The method is rapid,sensitive and completed within a single tube,without post-PCR handling of the amplification products.The entire reaction process takes about 30 minutes.This result provides an important reference for the detection and screening of A.rigidiuscula in the port.
万方数据
维普
