Identification of germplasm resources and analysis of phenotypic traits of the Auricularia delicata complex in China
Based on 27 strains of the Auricularia delicata complex collected from China,molecular identification was carried out by using ITS sequences,and 16 phenotypic characteristics were evaluated by means of analyses of diversity,correlation,cluster,and principal component.The results showed that the tested strains were identified as Auricularia sinodelicata and A.delicata of the Auricularia delicata complex.The Shannon-Wiener's diversity index of 16 phenotypic traits ranged from 0.42 to 1.60,with an average of 0.97;the variation coefficients of 4 quantitative characteristic scope were 4.35％to 26.49％;there is a correlation between some phenotypic characteristics.Cluster analysis indicated that 3 groups were divided at Euclidean distance of 1.54 in accordance with 5 phenotypic characteristics,namely,mycelium colloid,color change of mycelia(cultivated species),main color of ventral side of fresh basidiocarp,texture of basidiocarp and mature period of fruiting body.Principle component analysis changed 16 phenotypic characteristics into 5 principal components,and the cumulative contribution rate was 81.55％;the variance contribution rate of the first principal component was 26.67％,named as the fruit body color characteristic factor,and that of the second principal component was 23.05％,named as mycelium characteristic factor;the variance contribution rate of the third principal component was only 12.89％,defined as growth period-commodity characteristic factor.Through comprehensive analysis,three phenotypic characteristics,mycelium colloid,main ventral color of fresh basidiocarps and fruiting body mature period,were selected as the key indexes for germplasm evaluation.
the Auricularia delicata complexgermplasm resourcesphenotypic characteristicsdiversity
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