Molecular identification and microscopic morphological observation of the asexual stage of wild Pleurotus citrinopileatus
A wild mycelium sample growing on rotten stump of Pinuis yunnanensis collected from the Kunming Botanical Garden in Yunnan Province was identified as Pleurotus citrinopileatus by using molecular phylogenetic method.Morphological characteristics of asexual mycelium and spores were observed under stereomicroscope and light microscope.It was observed that asexual mycelium of P.citrinopileatus were dominated by dikaryotic hyphae with clamp connections and produced asexual spores in three ways:(1)spherical binucleate conidia with size ranging from 1.50 to 4.57 μm formed from short and unbranched conidiophores laterally differentiated from dikaryotic hyphae;(2)elliptical binucleate oidia with size ranging from 1.17 to 3.99 μm and cylindrical binucleate oidia with size being 2.78-5.40×1.57-3.03 μm formed from lateral branches of dikaryotic hyphae;(3)cylindrical uninucleate oidia with size being 3.71-6.70× 3.60-4.02 μm formed in chains from lateral monokaryotic branches of hyphae.The microscopic characters of asexual mycelium and spores were described and discussed in detail for the first time,with attached figures or drawing.
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