金针菇从原基期到伸长期要经历子实体的分化与发育,其分子机制仍不明确.本研究采用转录组和蛋白组联合分析,深入研究了金针菇Flammulina filiformis从原基期到伸长期子实体的发育机制.对比伸长期子实体与原基期,发现菌柄有1 220个差异基因和97个差异蛋白,菌盖有2 329个差异基因和387个差异蛋白.这些差异基因和蛋白在GO(gene ontology)功能聚类中主要涉及催化活性、细胞组分等.KEGG(kyoto encyclopedia of genes and genomes)富集分析显示,伸长期菌柄与原基期有105个差异基因分布于20条通路,其中19条为代谢通路;伸长期菌盖与原基期有163个差异基因分布于20条通路,其中18条为代谢通路.本研究依据蛋白组数据分析了差异蛋白互作网络,并随机选取8个差异表达基因进行实时荧光定量PCR验证.本研究为深入了解金针菇子实体生长发育机制提供了数据参考,为下一步研究金针菇及其他食用菌子实体分化与发育的分子调控机制筛选了候选功能基因.
Combined analyses of transcriptome and proteome during fruiting body development of Flammulina filiformis
Molecular mechanism of Flammulina filiformis fruiting body development and differentiation from primordium formation to stipe elongation stages remains unclear.In this study,combined analyses of transcriptome and proteome were carried out to reveal the expression patterns of genes and proteins during the development of Flammulina filiformis fruiting bodies from primordium formation to stipe elongation stages.Comparative analysis of the transcriptome and proteome in primordium and stipe elongation stages revealed that there were 1 220 differentially expressed genes(DEGs)and 97 differentially expressed proteins(DEPs)in the stipes,and 2 329 DEGs and 387 DEPs in the pileus.In the functional clustering of GO(gene ontology),these DEGs and DEPs mainly enriched in catalytic activity and cell components.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed that in primordium and elongated stipe 105 DEGs were annotated to 20 pathways in which 19 pathways were related to metabolism;in primordium and pileus of stipe elongation stage 163 DEGs were annotated to 20 pathways in which 18 pathways were related to metabolism.Additionally,the differential protein interaction network was analysed and eight differentially expressed genes were randomly selected for real-time quantitative PCR(RT-qPCR).In this study a data reference for further understanding the growth and development mechanism of Flammulina filiformis fruiting bodies was provided,and candidate functional genes were screened for further study of molecular regulatory mechanisms of fruiting body differentiation and development of Flammulina filiformis and other edible fungi.
Flammulina filiformisfruiting body developmenttranscriptomeproteomeprotein interaction network
NETL
NSTL
万方数据
