Comparative transcriptomics analysis of pathways and genes related to stipe elongation regulation of Flammulina filiformis
The stipe is the main edible part of Flammulina filiformis.The elongation position of the stipe is mainly located at the upper part,however,the regulation mechanism of stipe elongation is not well understood so far,and the related genes are rarely reported.In this study,the elongation characteristics of the upper,middle and lower parts of the stipe were analyzed,and the transcriptomic analysis and qRT-PCR verification were performed.The results showed that there were 532 DEGs in the middle part vs.lower part and 3 522 DEGs in the upper part vs.middle part of the stipe.GO function annotation of DEGs of upper part vs.middle part mainly included ribosome structure composition,structural molecular activity,transport activity and transmembrane transporter activity.KEGG analysis showed that up-regulated genes were significantly enriched in ribosome,steroid biosynthesis,DNA replication and other pathways,while down-regulated genes were significantly enriched in secondary metabolites and amino acid biosynthesis,glycolysis and other metabolic pathways.The DEGs with the highest difference multiples included chitinase,glycosyl hydrolase and transcription factors,etc.qRT-PCR results proved that the transcriptome data were accurate and reliable.This work comprehensively demonstrated the pathways and candidate genes that may be involved in the regulation of stipe elongation of F.filiformis,providing gene resources for subsequent gene function studies and molecular breeding of F.filiformis.
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