Functional study of gene Cdc24 regulating abiotic stress tolerance and fruiting body development in Flammulina filiformis
Biotic perception and response to abiotic stress can improve the environmental adaptability of organisms.Cdc24 gene is usually called guanylate exchange factor of small molecule G protein Rho family,involving in biological processes such as cell division,proliferation and differentiation.The function of Cdc24 gene in edible fungus Flammulina filiformis has not been studied.In this study,RNAi transformants of Cdc24 gene in F.filiformis were constructed by Agrobacterium-mediated genetic transformation.The results showed that the interference of Cdc24 gene significantly reduced the tolerance of mycelium to abiotic stresses such as salt,high temperature,low temperature,oxidation and acid-base.The polysaccharide and polyphenol content of RNAi transformant strains were lower than that of wild-type strains.The number of fruiting bodies and biological efficiency of RNAi transformant strains were also significantly lower than those of wild-type strains.This study shows that Cdc24 gene plays an important role in abiotic stress tolerance and fruiting body development of F.filiformis,and provides some data reference for variety improvement and efficient cultivation and production of F.filiformis.
Flammulina filiformisCdc24 geneabiotic stressfruiting body development
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