金针菇Cdc24基因参与调控非生物胁迫耐受性与子实体发育的功能研究
Functional study of gene Cdc24 regulating abiotic stress tolerance and fruiting body development in Flammulina filiformis
刘澳 1陈宇 2孙源 2戴鑫 2王庆佶 2王威2
作者信息
- 1. 山东农业大学植物保护学院山东省农业微生物重点实验室,山东泰安 271018;平邑县万寿宫林场,山东临沂 273300
- 2. 山东农业大学植物保护学院山东省农业微生物重点实验室,山东泰安 271018
- 折叠
摘要
生物感知和应答非生物胁迫的机制能提高其环境适应能力.Cdc24基因通常被称为小分子G蛋白Rho家族的鸟苷酸交换因子,参与细胞分裂、增殖和分化等生物学过程.Cdc24基因在食用真菌金针菇中的功能尚未研究.本研究通过农杆菌遗传转化构建了金针菇Cdc24基因的RNAi转化子菌株.结果表明,Cdc24基因被干扰后显著降低了菌丝对盐、高温、低温、氧化和酸碱等非生物胁迫的耐受性能力,RNAi转化子菌株的多糖和多酚含量均低于野生型菌株;RNAi转化子菌株的子实体数量、生物学效率指标也显著低于野生型菌株.本研究表明Cdc24基因在金针菇非生物胁迫耐受性和子实体发育过程中具有重要作用,为金针菇品种改良和高效栽培生产提供一定的数据参考.
Abstract
Biotic perception and response to abiotic stress can improve the environmental adaptability of organisms.Cdc24 gene is usually called guanylate exchange factor of small molecule G protein Rho family,involving in biological processes such as cell division,proliferation and differentiation.The function of Cdc24 gene in edible fungus Flammulina filiformis has not been studied.In this study,RNAi transformants of Cdc24 gene in F.filiformis were constructed by Agrobacterium-mediated genetic transformation.The results showed that the interference of Cdc24 gene significantly reduced the tolerance of mycelium to abiotic stresses such as salt,high temperature,low temperature,oxidation and acid-base.The polysaccharide and polyphenol content of RNAi transformant strains were lower than that of wild-type strains.The number of fruiting bodies and biological efficiency of RNAi transformant strains were also significantly lower than those of wild-type strains.This study shows that Cdc24 gene plays an important role in abiotic stress tolerance and fruiting body development of F.filiformis,and provides some data reference for variety improvement and efficient cultivation and production of F.filiformis.
关键词
金针菇/Cdc24基因/非生物胁迫/子实体发育Key words
Flammulina filiformis/Cdc24 gene/abiotic stress/fruiting body development引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据