摘要
双单杂交是担子菌类食用真菌重要的杂交育种手段,目前对双单杂交中先导核的研究不够充分.为了探究双单杂交中先导核的遗传学规律,本研究以斑玉蕈3株双核体菌株B3、X3和NN12与 9 株单核体菌株H3-8、H4-4、H4-10、H13-3、H13-6、H14-10、NN12-1、X3-1A和X3-13 为供试菌株,进行完全亲和双单杂交,并开发交配型分子标记鉴定先导核.结果表明,以B3、X3和NN12为核供体的双单杂交实验中,分别获得新的杂交双核体89、87和75株.交配型分子标记鉴定结果显示B3、X3和NN12核供体中两个细胞核成为先导核的比例分别为41:48、44:43和36:39,均符合统计学1∶1的比例,说明在斑玉蕈供试双核体菌株中两个细胞核作为先导核的概率相同.
Abstract
Dikaryon-monokaryon(di-mon)mating is an important breeding method of edible basidiomycete mushrooms.Currently,research on the leading nucleus in di-mon mating is not sufficient.In this study,the genetic patterns of the leading nuclei in di-mon mating were explored.A complete compatibility di-mon mating test was conducted by using three Hypsizygus marmoreus dikaryon strains,B3,X3,and NN12,and nine monokaryon strains,H3-8,H4-4,H4-10,H13-3,H13-6,H14-10,NN12-1,X3-1A,and X3-13,as tested strains.Molecular markers based on mating type genes were developed to identify the leading nuclei.The results showed that hybridization pairs using B3,X3,and NN12 as the nuclear donors obtained 89,87,and 75 di-mon mating hybrid strains,respectively.The results of mating type molecular marker identification showed that the ratio of the two nuclei becoming leading nuclei in the nuclear donors B3,X3,and NN12 were 41:48,44:43 and 36:39,respectively,all conforming to the statistical ratio of 1∶1,indicating that the probability of the two nuclei in the tested dikaryotic strains of H.marmoreus serving as leading nucleus is the same.
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