Genetic patterns of dikaryon nuclei becoming leading nuclei in the dikaryon-monokaryon(di-mon)mating of Hypsizygus marmoreus
Dikaryon-monokaryon(di-mon)mating is an important breeding method of edible basidiomycete mushrooms.Currently,research on the leading nucleus in di-mon mating is not sufficient.In this study,the genetic patterns of the leading nuclei in di-mon mating were explored.A complete compatibility di-mon mating test was conducted by using three Hypsizygus marmoreus dikaryon strains,B3,X3,and NN12,and nine monokaryon strains,H3-8,H4-4,H4-10,H13-3,H13-6,H14-10,NN12-1,X3-1A,and X3-13,as tested strains.Molecular markers based on mating type genes were developed to identify the leading nuclei.The results showed that hybridization pairs using B3,X3,and NN12 as the nuclear donors obtained 89,87,and 75 di-mon mating hybrid strains,respectively.The results of mating type molecular marker identification showed that the ratio of the two nuclei becoming leading nuclei in the nuclear donors B3,X3,and NN12 were 41:48,44:43 and 36:39,respectively,all conforming to the statistical ratio of 1∶1,indicating that the probability of the two nuclei in the tested dikaryotic strains of H.marmoreus serving as leading nucleus is the same.
Hypsizygus marmoreusdi-mon matingleading nucleusmating type molecular markers
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