Regulatory behavior of vegetative compatibility in Morchella importuna
Vegetative incompatibility(Ⅵ)is ubiquitous between single-ascospore isolates originated from the same ascocarp of Morchella importuna.In this study,ascocarps of M.importuna with different genetic background were used as materials for obtaining single-ascospore isolates from the same asci,and the vegetative compatibility relationship between the isolates originated from the same asci and different asci were analyzed.Mating type and the genotype of mimpvic32 and mimpvic33 of the isolates were identified.The results showed that no more than four vegetative compatibility groups(VCGs)were divided among the offsprings of YPL6 populations.Parent Y282 and Y346 were vegetatively compatible,and eight isolates originated from the spores of same asci of the progeny ascocarps were vegetatively compatible,and all the progeny single-ascospore isolates were compatible and formed a VCG with the parent.Hybridization were performed between Y282 and Y134 showing type Ⅰ barrage,and between Y282 and Y88 showing type Ⅱ barrage,and eight isolates originated from spores of the same asci of the progeny ascocarps formed 2-3 VCGs.When Y282 crossed with the wild isolates ZQW10 and ZQW31 respectively,eight isolates originated from spores of the same asci of the progeny ascocarps were divided into 4 VCGs,and the single-ascospore isolates originated from different asci of the same ascocarp were generally incompatible and produced more VCGs.It is deduced that the Ⅵ is controlled by multiple loci in M.importuna,and all the progeny isolates become a VCG when there are no genetic differences in the Ⅵ regulatory loci between parents.The more the numbers of regulatory loci with genetic difference exist between parents,the more the numbers of VCG are formed by the progeny single-ascospore isolates.During spore development in an ascus,the ways of chromosome recombination,exchange and segregation are different at the stage of meiosis,and genetic and phenotypic differences produce between spores.
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