Biological characteristics and agronomic traits of a wild Morchella sextelata strain from Zhouqu,Northwest China
A wild Morchella strain MS-1 was isolated from an ascocarp collected from high-altitude areas in Zhouqu County,Gansu Province.Identification based on multi-gene analysis of ITS,RPB1,RPB2 and EF1-α proved that this wild strain was M.sextelata.The optimal temperature for the growth of wild strain MS-1 during the stock culture was 18-20 ℃;the minimum growth temperature was 1 ℃,and the maximum 33 ℃.The optimal pH value is 6-7.The optimal carbon source is lactose and soluble starch.The optimal nitrogen source is yeast extract and beef dipping powder.The optimal C/N ratio is 40:1-45:1.The lethal temperature under 2 h high-temperature treatment during stock culture is 44 ℃,while that during expanded cultivation is 48 ℃;the yield per unit area is 0.6 kg/m2,significantly higher than that of commercial production strains using for comparison.Its agronomic traits are excellent,with a medium cultivation period of 140 d.Comprehensive analysis shows that the wild M.sextelata MS-1 has potential to be further developed as a new commercial production strain.
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