摘要
以甘肃省舟曲县高海拔地区采集的一株野生羊肚菌菌株MS-1为试验材料,进行ITS、RPB1、RPB2、EF1-α多基因鉴定,对其菌丝体阶段及子实体阶段生物学特性进行研究,并与7个商业生产羊肚菌菌株进行比较试验.研究表明:野生菌株MS-1为六妹羊肚菌Morchella sextelata.MS-1母种阶段生长最适温度为18-20 ℃,最低温度为1 ℃,最高温度为33 ℃,最适pH值为6-7,最适碳源为乳糖和可溶性淀粉,最适氮源为酵母浸粉和牛肉浸粉,最适C/N比为40∶1-45∶1;该菌株较耐高温,母种阶段高温处理2 h得出致死温度为44 ℃,栽培种阶段高温致死温度为48 ℃;单位面积产量为0.6 kg/m2,显著高于5个商业生产菌株,农艺性状较好,栽培周期中等,为140 d.综合分析表明,野生羊肚菌MS-1具备作为商业生产菌株进一步开发的潜力.
Abstract
A wild Morchella strain MS-1 was isolated from an ascocarp collected from high-altitude areas in Zhouqu County,Gansu Province.Identification based on multi-gene analysis of ITS,RPB1,RPB2 and EF1-α proved that this wild strain was M.sextelata.The optimal temperature for the growth of wild strain MS-1 during the stock culture was 18-20 ℃;the minimum growth temperature was 1 ℃,and the maximum 33 ℃.The optimal pH value is 6-7.The optimal carbon source is lactose and soluble starch.The optimal nitrogen source is yeast extract and beef dipping powder.The optimal C/N ratio is 40:1-45:1.The lethal temperature under 2 h high-temperature treatment during stock culture is 44 ℃,while that during expanded cultivation is 48 ℃;the yield per unit area is 0.6 kg/m2,significantly higher than that of commercial production strains using for comparison.Its agronomic traits are excellent,with a medium cultivation period of 140 d.Comprehensive analysis shows that the wild M.sextelata MS-1 has potential to be further developed as a new commercial production strain.
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