Biological characteristics and domestication cultivation of Sparassis subalpina
The fruiting bodies of a wild Sparassis species collected from Milin,Xizang,were identified on the basis of morphology and molecular analysis as Sparassis subalpina.The pure mycelium(XQ-1)was isolated from the fruiting body by tissue isolation method.The optimal stock culture medium was screened,and biological characteristics and domestication of the strain were studied.The most suitable stock culture medium for XQ-1 strain was carrot pine sawdust dextrose agar(CSDA)medium.Single factor experiments showed that the optimal carbon and nitrogen sources for mycelium growth were glucose and malt powder,respectively,and the optimal carbon-nitrogen ratio was 5:1 and 10:1.The optimal pH was 6.0-6.5;the optimal inorganic salt was magnesium sulfate;adding vitamin C and vitamin B1(respectively 20 mg/L)to the medium could stimulate the mycelium growth;the optimal growth temperature was 21 ℃.Orthogonal experiments showed that fructose 20.0 g/L,malt extract 2.0 g/L,pH 6.5 and magnesium sulfate 0.1 g/L were the best medium combinations.The highest enzyme activity of hydroxymethyl cellulase was 191.62 U/mL.Adding wheat flour,barley flour and corn flour to the cultivation substrate could successfully speed up fruiting body production,and peanut flour,barley flour and glutinous rice flour could play a positive role in the growth of mycelium.
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