摘要
本研究结合形态学特征及分子数据将唐山市迁西县板栗栽培区采集到的一株野生多孔菌XHMK鉴定为血红栓孔菌Trametes sanguinea.通过单因素试验探讨了不同因素对该菌株菌丝生长的影响.菌丝生长的最佳碳源、氮源、无机盐分别为果糖、酵母浸粉、磷酸二氢钾,最适pH为7,最适温度为35 ℃.正交试验表明,该菌株菌丝最佳生长条件组合为果糖25 g/L、酵母浸粉2 g/L、磷酸二氢钾1 g/L、pH为6.5.驯化试验的优化栽培料为栗木屑70％、玉米芯10％、麸皮19％、石膏1％,发菌期为36 d,生物转化率为9.49％.
Abstract
A wild polypore strain XHMK collected from chestnut cultivation area in Qianxi County,northern China,was identified as Trametes sanguinea based on morphological features and molecular data.The effects of different factors on the mycelial growth of this strain were explored through single factor experiments.The optimal carbon source,nitrogen source,and inorganic salt for the mycelial growth were fructose,yeast extract powder,and potassium dihydrogen phosphate,respectively.The optimal pH and temperature for the mycelial growth were 7 and 35 ℃,respectively.The orthogonal experiment showed that the optimal combined conditions of mycelium growth for this strain were fructose 25 g/L,yeast extract 2 g/L,potassium dihydrogen phosphate 1 g/L,and pH 6.5.The optimized cultivation substrates for domestication were 70％chestnut sawdust,10％corn cob,19％bran,and 1％gypsum.It took 36 days to fulfill the cultivation period,and the biological efficiency was 9.49％.
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