A wild polypore strain XHMK collected from chestnut cultivation area in Qianxi County,northern China,was identified as Trametes sanguinea based on morphological features and molecular data.The effects of different factors on the mycelial growth of this strain were explored through single factor experiments.The optimal carbon source,nitrogen source,and inorganic salt for the mycelial growth were fructose,yeast extract powder,and potassium dihydrogen phosphate,respectively.The optimal pH and temperature for the mycelial growth were 7 and 35 ℃,respectively.The orthogonal experiment showed that the optimal combined conditions of mycelium growth for this strain were fructose 25 g/L,yeast extract 2 g/L,potassium dihydrogen phosphate 1 g/L,and pH 6.5.The optimized cultivation substrates for domestication were 70％chestnut sawdust,10％corn cob,19％bran,and 1％gypsum.It took 36 days to fulfill the cultivation period,and the biological efficiency was 9.49％.
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