Domestication and cultivation of a wild Pholiota limonella strain
Domestication and cultivation studies of wild Pholiota limonella strains collected from Shandong Province were carried out.Formulation consisting of 40％of cottonseed shell,40％of wood chips,10％of bran,8％of corn flour and 2％of gypsum powder was suitable for artificial cultivation.By using this formula,the biological efficiency of the first two crops was 54％,and the biological efficiency increased by 7.2％,reaching 57.90％,after three consecutive generations of domestication.The number of fruiting bodies per cluster increased by 1.66 times as against the original wild strain.The commodity character is good,and the domestication cultivation effect is remarkable.The biological efficiency of the top fruiting mode of bag-cultivation was 56.30％,significantly higher than that of the porous fruiting mode in perforated bag.The results of orthogonal experiment showed that the four factors tested had significant effects on the biological efficiency of P.limonella,among which the combination of 50％of Lentinula edodes residue,20％of wheat bran,2％of shell powder,1％of gypsum powder and 27％of wood chips was the optimized alternative formula.Nutrient analysis of fruit body protein and amino acid showed that the protein content was 35.17％and the amino acid scored 64.7 points,and umami amino acid content accounted for 44.52％of the total amino acid,indicating high nutritional value.It is considered that P.limonella is a rare edible fungus with great development potential,and this study provide reference for artificial cultivation and application of this mushroom.
wild germplasm resourcesdevelopment and utilizationPholiota limonelladomesticated cultivationnutritional evaluation
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