Chemical constituents from fruiting body of Phellodon cinereofuscus and their inhibitory activities against α-glucosidase
Diabetes is one of the most common chronic diseases,capable of generating various complications.α-Glucosidase serves as a crucial therapeutic target for diabetes,and the exploration of novel α-glucosidase inhibitors holds significant importance for diabetes treatment.The chemical constituents from fruiting body of Phellodon cinereofuscus and their inhibitory activities against α-glucosidase were studied.Sixteen compounds from 70％ethanol extract of P.cinereofuscus were isolated and purified by silica gel column,HP20 column chromatography,ODS column chromatography and high performance liquid chromatography(HPLC).The structures of them were identified by nuclear magnetic resonance(NMR)as(22E,24R)-3β-hydroxy-24-methylcholesta-5,22-dien-7-one(1),(22E,24R)-ergosta-4,6,8(14),22-tetraen-3,15-dion(2),5α,8α-epidioxyergosta-6,9,22-trien-3β-ol(3),(22E,24S)-cerevisterol(4),20R,24R,9α-hydroxy-Δ4,6,8(14),22-tetraene-3,15-dione-ergosterol(5),glaucoposterol B(6),(22E,24R)-5α,6α-epoxyergosta-8(14),22-diene-3β,7α,15α-triol(7),3β-Hydroxyl-(22E,24R)-ergosta-5,8,22-trien-7,15-dione(8),5α,6α-epoxy-(22E,24R)-ergosta-8(14),22-diene-3β,7α-diol(9),(22E,24R)-5α,6α-epoxy-ergosta-8,22-dien-3β,7α-diol(10),demethylincisterol A3(11),betulinic acid(12),lup-20(29)-en-3α,28-diol(13),(Z)-6-nonadecenoic acid(14),tetradecanoic acid(15),hexacosane(16).All the compounds were obtained from the genus of Phellodon for the first time.The inhibitory activities of compounds 1-16 against α-glucosidase were evaluated.Compounds 3,6,11,13 and 14 showed significant inhibitory activity against α-glucosidase with IC50 values of(19.51±5.68),(6.29±1.16),(12.15±2.21),(6.81±0.26),(10.26±1.84)μmol/L respectively.The results provide theoretical support for further research of the medicinal function of P.cinereofuscus.
Phellodon cinereofuscuschemical constituentsstructure identificationinhibitory activity against α-glucosidase
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