Domestication and antioxidant activities of Fulvifomes nonggangensis
Single factor and orthogonal experiments were conducted on the biological characteristics of the wild strain of Fulvifomes nonggangensis collected from Nonggang Nature Reserve in Guangxi,and domestication cultivation of the fungus was attempted.Preliminary research was also conducted on the antioxidant activity of water extract of the cultivated fruiting bodies.The results showed that the optimal combination of culture condition was fructose as the carbon source,yeast extract as the nitrogen source,culture temperature of 28 ℃,and pH value of 6.5;temperature had the greatest influence on mycelial growth.Domestication cultivation experiment indicated that formulation of cottonseed hulls 40％,sawdust 38％,bran 20％,lime 1％,and gypsum 1％was the best combined substrate,displaying colonial growth rate of 0.33 cm/d,average yield of 27.26 g/bag,and biological efficiency of 12.98％.Water extract of fruiting body was obtained by using ultrasonic method.When the extract concentration was up to 5 mg/mL,the clearance rate of DPPH reached 84.45％,when it was only 2.5 mg/mL,the clearance rate of ABTS still reached 93.82％,indicating fairly good antioxidant activity of the fungus.This data hopefully gives support to the large-scale cultivation and industrial utilization of F.nonggangensis in future.
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