提高黑色素得率的黑木耳发酵配方优化及黑色素的理化性质研究
Optimization of fermentation medium formula for increasing melanin yield and physicochemical properties of melanin of Auricularia heimuer
姚秀鸽 1郭燕 1韩闯 1田爽 1朱加楠 1周舒扬 1刘佳宁 1戴肖东 1张丕奇 1马庆芳 1张介驰 1马银鹏1
作者信息
- 1. 黑龙江省科学院微生物研究所,黑龙江哈尔滨 150010
- 折叠
摘要
为提高黑木耳发酵液中黑色素得率,本研究以黑木耳1703为试验菌株,采用单因素实验和响应面法优化发酵培养基配方,研究黑木耳黑色素的光、热和金属离子稳定性及体外抗氧化活性.结果表明:蔗糖、蛋白胨、MgSO4对黑木耳发酵液中黑色素得率影响显著,最佳发酵培养基配方为马铃薯200 g/L、蔗糖21.40 g/L、蛋白胨5.34 g/L、MgSO4 0.56 g/L,在此条件下黑木耳黑色素得率为0.050 g/100 mL;黑木耳黑色素具有良好的光、温度及金属离子稳定性,色素保存率均在90％以上;黑木耳黑色素具有较强的DPPH自由基、超氧阴离子自由基清除能力和铁还原力,其中对DPPH自由基、超氧阴离子自由基的IC50值分别为0.021 mg/mL和0.90 mg/mL.本研究为黑木耳黑色素的液体发酵制备及应用提供理论参考.
Abstract
For increasing the melanin yield in Auricularia heimuer fermentation broth,fermentation medium formula of the experimental strain A.heimuer 1703 was optimized by single factor experiments and response surface methodology.The stability and antioxidant activities of melanin were determined.The results showed that sucrose,peptone,and MgSO4 had a significant impact on the melanin yield of A.heimuer fermentation broth.The optimal fermentation medium for increasing A.heimuer melanin yield was potato of 200 g/L,sucrose of 21.40 g/L,peptone of 5.34 g/L,and MgSO4 of 0.56 g/L.Under these conditions,the melanin yield was 0.050 g/100 mL.A.heimuer melanin has good stabilities under different illumination condition and temperature,and in solution containing different metal ions,showing pigment retention rate of over 90％.A.heimuer melanin has strong DPPH free radical,superoxide anion free radical scavenging abilities,and iron reducing ability.The IC50 values for DPPH free radical and superoxide anion free radical were 0.021 mg/mL and 0.90 mg/mL,respectively.This study provides theoretical reference for the liquid fermentation production and application of A.heimuer melanin.
关键词
黑木耳/黑色素/液体发酵/响应面法/理化性质/抗氧化Key words
Auricularia heimuer/melanin/liquid fermentation/response surface methodology/physical and chemical properties/antioxidant引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据