Analysis of Mating-type Loci of Monokaryotic Strains in Ganoderma leucocontextum
Due to its medicinal value,Ganoderma leucocontextum,a subspecies of Ganoderma carrying regional characteristics,has attracted much attention.However,the structure of its mating-type loci still remains unknown.In this study,whole genomes of two monokaryotic strains,GL72 and GL81,were sequenced by using the next-generation sequencing and single-molecule real-time sequencing technologies.Based on the genomic information,sequences and structure of mating-type loci of the above two monokaryotic strains were extensively analyzed.Our results revealed clearly that the mat A locus of both strains has one pair of HD genes with a head-to-head direction,which is similar to that of the most basidiomycetes.Within the HD1 gene region,however,it was found that strain GL72 contains two bi-type nuclear localization signal domains and HD1-specific sequences as well while strain GL81 holds only one bi-type nuclear localization signal domain.There also existed some significant differences within the coding sequences of homologous α-helix Ⅰ domain in HD2 gene between the two strains.Genes flanking the mat A locus varied significantly,moreover,between the two monokaryotic strains.Furthermore,the mat B loci of both strains of GL72 and GL81 were found to carry the same number of eight pheromone receptor genes,but strain GL72 had nine putative pheromone precursor genes while strain GL81 had only five.The above differences discovered from the mating-type genes in the compatible monokaryotic strains of G.leucocontextum in this study should provide a theoretical foundation for our genetic studies and Ganoderma breeding in future.
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