Study on the Quality Standard of Sophora Tonkinensis Gagnep Extract
The quality standard of the extract from the Sophora tonkinensis Gagnep was established.Ethanol concentration,solid-liquid ratio,reflux time and extraction times were selected as the influencing factors.Orthogonal experiment was used to select the extraction technology of the Sophora tonkinensis Gagnep,and the content of matrine and oxymatrine in the Sophora tonkinensis Gagnep was determined by HPLC.Orthogonal test results showed that the optimal extraction process was as followings:ethanol concentration(was 60％),solid-liquid ratio was 1:20(g:mL),reflux time was 2.0 h,extraction times were 3 times.HPLC results showed that matrine and oxymatrine had good linear relationship in the selected range,and the recovery RSD was not higher than 2％.The extraction process is stable and feasible,and the established HPLC method is simple and fast.
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