Chemical composition of five different chemotype essential oils from camphor tree was tested using GC and GC-MS methods.The results indicated that the high content components among the five chemotype of essential oils were linalool(91.98%),camphor(81.25%),borneol(91.88%),trans-nerolidol(52.63%)and eucalyptol(57.73%).Moreover,pancreatic lipase inhibitory activity of different chemotype essential oils was also studied.The pancreatic lipase inhibition activity of essential oils showed that the comparison of chemotype was as follows:trans-nerolidol chemotype>eucalyptol chemotype>linalool chemotype>camphor chemotype>borneol chemotype.The IC50 value of trans-nerolidol chemotype was(20.02±3.27)mg/mL,and the enzyme inhibition ratio can reach up to 90.17%±1.56%on concentration of 100 mg/mL.