Research on the Quality Standard for the Seeds of Thermopsis lanceolata R.Br.
To establish a quality standard for the seeds of Thermopsis lanceolata R.Br.(STL),water content,total ash,and extractives of STL were determined according to the Pharmacopoeia of the Peoples's Republic of China(2020 edition,volume 4),microscopic characteristics were studied based on its powder.A TLC method was established.The HPLC method for the determination of cytisine,baptifoline,N-methylcytisine and thermopsine in STL were developed and validated with four indicators as the reference substance.Microscopic characteristics of the powder as well as the TLC spots were clear.The average for the water,total ash,acid insoluble ash,and extractives were 8.10% ,3.13% ,0.18% ,and 33.29% ,respectively.The linearities of cytisine,baptifoline,N-methylcytisine and thermopsine were good in the selected range(R2=0.999 9),the average recoveries were 101.1% (RSD of 0.53% ),94.0% (RSD of 0.78% ),99.3% (RSD of 0.67% ),and 102.4% (RSD of 0.64% ),respectively;and the average contents were 1.86% ,0.25% ,0.10% ,and 0.19% .This method provides evidence for the scientific evaluation of the quality standards of STL.
NETL
NSTL
万方数据
