Studv on the Detection and Analysis Method of Aspoxicillin Polvmer
In order to establish a method for the determination of apocycillin polymer and in order to study its methodology,the method was determined by HPLC.The detection conditions of liquid chromatography were as follows:the column was composed of Dextran G-10 column(40～120 μam)as filler,the inner diameter of the glass column was 1.4 cm,the column length was 40 cm,and 0.2 mol/L phosphate buffer[0.2 mol/L Na2HPO4 solution-0.2 mol/L NaH2PO4 solution(95∶5)]at pH 8.0 was used as mobile phase A,water was used as mobile phase B,the flow rate was determined to be 1.0 mL/mL,and the detection wavelength was set at 254 nm.The concentration ranged from 20.052 mg/ml to 40.225 mg/mL,and the peak area ratio showed a good linear relationship(A=25.96.C-2.55× 102).The LOD was 0.8 µg,and the LOQ was 2.0 µg.The method is simple,accurate and suitable for the determination of aspoxicillin polymer.
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