多指标响应面优化枸杞提取工艺研究
Research on the Extraction Process of Lycium barbarum with Multi-index Response Surface Optimization
杜蓓 1李艺 1杨长花 1庞亚蓉1
作者信息
- 1. 陕西国际商贸学院,陕西 西安 712046
- 折叠
摘要
枸杞中含有丰富的枸杞多糖和枸杞黄酮,均具有较高的药用价值.选取提取方法、提取时间、料液比、提取次数、提取温度、浸泡时间进行单因素试验,根据单因素结果选取3个影响较大的因素,结合响应面法中的Box-Behnken试验设计对枸杞总多糖和总黄酮的提取工艺进行优化,得到优化提取工艺条件为:加热回流法,浸泡1.0h,提取时间55 min,料液比1∶12(g∶mL),提取温度81℃,提取2次.总多糖提取率能够达到45.36％,总黄酮提取率达到0.29％.该方法提取率高、重复性好,高效环保.为枸杞中总多糖和总黄酮的提取方法提供参考依据.
Abstract
Lycium barbarum is rich in Lycium barbarum polysaccharide(LBP)and Lycium barbarum flavones,both of which possess significant medicinal value.The effects of extraction method,extraction time,solid-liquid ratio,extraction times and extraction temperature and snaking time were studied by single factor test.Based on the results of single-factor experiments,three significant effects were selected to optimize the extraction technology of LBP and Lycium barbarum flavones using Box-Behnken experimental design.The optimal extraction conditions are determined through these experimental results:by the heating and reflux method,soaking for 1.0 h,55 minutes of extraction time,a solid-liquid ratio of 1∶12(g∶mL),extraction temperature of 81 ℃,and extracting twice.Under these conditions,the extraction rates of LBP and Lycium barbarum flavones reached 45.36％and 0.29％,respectively.The method has high extraction rate,good reproducibility,high efficiency and environmental sustainability,providing a valuable reference for the extraction method of LBP and Lycium barbarum flavones from Lycium barbarum.
关键词
枸杞多糖/枸杞黄酮/响应面优化/提取率Key words
LBP/Lycium barbarum flavones/response surface optimization/extraction rate引用本文复制引用
基金项目
陕西省教育厅重点科研计划协同创新中心项目(2023)(23JY006)
出版年
2024
NETL
NSTL
维普
万方数据