The Separation and Purification Method of Pyrrole from Fermentation Broth
Pyrrole is an important raw material in the modern chemical and pharmaceutical industries,and it is valuable to explore the potential purifying technique of Pyrrole biosynthesis.Two separation methods,extraction and distillation,were used,mainly focusing on the selection of extractants,optimization of extraction conditions,and refinement of distillation parameters.The purified Pyrrole was analyzed by high-performance liquid chromatography and mass spectrometry.The optimal purification conditions were determined as follows:Extracted with dichloromethane,extracted three times at room temperature,distilled at 25 ℃ with a vacuum degree of 0.07 MPa.Under these conditions,the yield of high purified Pyrrole was 94.4％.
Pyrrolefermentation brothdichloromethaneextractiondistillation under reduced pressure
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