三重四级杆液质联用测定乳酸环丙沙星中哌嗪的残留量
Determination of Piperazine in Ciprofloxacin Lactate by Triple Quadrupole Liquid Mass Spectrometry
杨帅 1李欣 1于亮 1李霞 1杨海霞1
作者信息
- 1. 山东省药学科学院,山东 济南 250101;国家药监局药物制剂技术研究与评价重点实验室,山东 济南 250101
- 折叠
摘要
建立液质联用法测定乳酸环丙沙星中哌嗪残留量的方法.采用C18色谱柱分离,串联四级杆质谱多反应离子监测方式检测,外标法定量.哌嗪浓度在0.021～1.28 μg/mL范围内与峰面积线性关系良好(r=0.998),平均回收率为98.9％(RSD=1.61％).方法专属性强、重复性好、灵敏度高,可用于乳酸环丙沙星中哌嗪残留量测定.
Abstract
To establish a method for the determination of piperazine in ciprofloxacin lactate by liquid mass spectrometry,samples were separated by C18 chromatographic column,detected by tandem quadrupole mass spectrometry multi-reactive ion monitoring,and quantified by external standard method.A good linear relationship was obtained in the concentration range of 0.021～1.28 μg/mL for piperazine,with the correlation efficiency of 0.998.The average recovery was 98.9％(n=6),with an RSD of 1.61％.This method has good specificity,repeatability and high sensitivity.It can be used for the determination of residual piperazine in ciprofloxacin lactate.
关键词
液质联用/哌嗪/乳酸环丙沙星Key words
LC-MS/piperazine/ciprofloxacin lactate引用本文复制引用
出版年
2024
NETL
NSTL
万方数据