摘要
采用分段减压蒸馏结合柱层析方法,以目标成分含量大于80.0％为目标,开展了樟树精油中＜10.0％低含量成分的高效分离研究,获得了柠檬烯(93.2％)、乙酸龙脑酯(87.5％)、反式-橙花叔醇(85.8％)、β-石竹烯(91.1％)、4-松油醇(92.6％)、α-松油醇(87.2％)等6种组分,收率分别为 36.0％、14.0％、11.0％、21.0％、38.0％和10.0％.进一步研究了各组分的抗氧化性能,结果表明,乙酸龙脑酯、反式-橙花叔醇、4-松油醇和α-松油醇组分的IC50值分别为96.6、87.6、73.6和105.4 mg/mL,抗氧化性能优于单萜标准品;柠檬烯和β-石竹烯组分的IC50值分别为66.2和48.5 mg/mL,抗氧化性能低于单萜标准品.
Abstract
A method of staged vacuum distillation combined with column chromatography was developed on highly performance separation of six low content components with the initial content less than 10.0％to more than 80.0％from camphor tree essential oils,in which the six compounds included limonene(93.2％),bornyl acetate(87.5％),trans-nerolidol(85.8％),β-caryophyllene(91.1％),4-terpineol(92.6％),and α-terpineol(87.2％)were obtained by flash chromatography column with the yield of 36.0％,14.0％,11.0％,21.0％,38.0％,and 10.0％,respectively.Furthermore,the antioxidant properties of each fraction and reference compoundswere evaluated.The IC50 values of bornyl acetate,trans-nerolidol,4-terpineol and α-terpineol fraction were 96.6 mg/mL,87.6 mg/mL,73.6 mg/mL,and 105.4 mg/mL,respectively,which exhibited superior antioxidant properties compared to corresponding reference compound(monoterpenes).The IC50 values of limonene and β-caryophyllene fraction were 66.2 mg/mL and 48.5 mg/mL,respectively,which exhibited lower antioxidant properties than the corresponding reference compound.
基金项目
江西省科技厅重大科技研发专项(20203ABC28W016)
维普
万方数据