The simultaneous determination of morpholine and pyridine by UPLC MS/MS was established.The chromatographic column C18(4.6 mm× 100 mm,2.6 µm)was used.Isocratic elution was performed with 5 mmol/L ammonium-formic acid aqueous solution(A)-acetonitrile(B)as the mobile phase.The flow rate was 0.6 mL/min and the column temperature was 30 ℃.The injection volume was 5 μL.The quantitative ion pairs were morpholine 88/70.2 and pyridine 79.9/53,and the detection mode was multiple reaction monitoring(MRM).The method is simple,accurate and sensitive,and can accurately determine the content of morpholine and pyridine simultaneously.
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