Determination of Encapsulation Efficiency of Tamoxifen Citrate Liposomes by Microcolumn Centrifugation-HPLC Method
A method to determine the encapsulation efficiency of Tamoxifen citrate liposomes(TAM-LIPs)was established.TAM-LIPs were prepared by thin film dispersion method.The microcolumn was filled with Sephadex G-50 gel and water was used as the elution.After separation of TAM-LIPs and free drugs by centrifugation,the concentration of tamoxifen was determined by high performance liquid chromatography(HPLC)and the encapsulation efficiency(EE)was calculated.Then the experimental condition of microcolumn centrifugation method was optimized by Box-Behnken design.The drug content could be accurately determined by HPLC.The elution curve showed that the drug-loaded liposomes could be completely eluted for 4 times.Under the optimal conditions,the liposomes and free drugs could be separated to the ultmost extent.The RSD of three batches of TAM-LIPs were verified to meet the methodological requirements.The established microcolumn centrifugation-HPLC method was accurate and simple,which could be used to determine the EE of TAM-LIPs.
万方数据
维普
