Study on HPLC Fingerprint of Liuwei Dihuang Pills Chinese Medicine Fine Powder
A HPLC fingerprint detection method of Liuwei Dihuang Pills Chinese medicine fine powder is established,providing a quality research method for the improvement of the process of Liuwei Dihuang Pills.Using high performance liquid chromatography,the chromatographic column is filled with octadecylsilane bonded silica gel(C18,4.6 mm×250 mm,5 μm),the mobile phase is acetonitrile(A)and 0.08％phosphoric acid aqueous solution(B),the flow rate is 1.0 mL/min,the detection wavelength is 238 nm,the column temperature is 30 ℃,the injection volume is 10 μL.The similarities between the fingerprint spectra of the 16 batches of samples and the fingerprint spectra under standard conditions are greater than 0.99.The method has good specificity and durability,and can be used for the fingerprint determination of Liuwei Dihuang Pills Chinese medicine fine powder.
Liuwei Dihuang Pills Chinese medicine fine powderhigh performance liquid chromatographyfingerprint spectrum
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