Research Advances on Primary Culture of Daphnis nerii Embronic Cell in Vitro
Daphnis nerii(Linnaeus)is a notorious pest that has a wide geographic distribution.In this study,we employed a mechanical crushing method to establish primary cultures of Daphnis nerii eggs and determined Grace's insect medium as the most optimal culture medium.Primary culture of Daphnis nerii embryonic cells lasted up to 12 months,and various forms of primary cells were differ-entiated in the primary culture.Our result provides crucial experimental basis for establishing Daph-nis nerii cell lines in future research.
Daphnis neriiprimary culturemorphological observationinsect cell line
国家科技期刊平台
NSTL
万方数据
维普
