土壤四环素类抗生素前处理方法研究
Study on Soil Pretreatment Methods of Tetracycline Antibiotics
赵佳文 1肖满成 1余晓玲 1曾芳发 1乔支卫1
作者信息
- 1. 江西省生态环境监测中心,330046,南昌
- 折叠
摘要
为建立测定土壤四环素类抗生素振荡萃取、冷冻分离、小柱净化的前处理方法,通过对土壤中 3 种四环素类抗生素的提取方式、萃取溶剂类型、净化方法和提取液保存条件等前处理技术进行研究,使用液相色谱串联质谱仪对四环类抗生素进行测定.使用该方法不同浓度水平的四环素抗生素回收率在 80.0%～118.0%,相对标准偏差小于 20%,该前处理方法适应性强、操作简单、提取效率较高,适用于土壤中四环素类抗生素残留分析.
Abstract
In order to establish a pre-treatment method for the determination of tetracycline antibiot-ics in soil,including shaking extraction,freezing separation,and small column purification,the pre-treatment techniques of three tetracycline antibiotics in soil,including extraction methods,extrac-tion solvent types,purification methods,and preservation conditions of the extraction solution,were studied.The tetracycline antibiotics were determined using a liquid chromatography tandem mass spectrometer.The recovery rates of tetracycline antibiotics at different concentration levels using this method range from 80.0%to 118.0%,with a relative standard deviation of less than 20%.This pre-treatment method has strong adaptability,simple operation,and high extraction efficiency,making it suitable for the analysis of tetracycline antibiotic residues in soil.
关键词
四环素类/振荡提取/冷冻分离/液相色谱串质谱仪Key words
tetracyclines/vibrating extraction/freezing separation/liquid chromatography tandem mass spectrometer引用本文复制引用
基金项目
生态环境部长江流域生态环境监督管理局项目(YZJ-2022-025)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据