Study on Micro-cuttage Propagation of Cinnamomum Porrectum ct.Citral
To simplify the seedling raising procedure of plantlets and realize the large-scale production of fine varieties of C.porrectum,the study focuses on the effects of acclimatization time,IBA concentration and cutting medium on rooting of C.porrectum ct.citral,taking tissue culture plantlets of Cinnamomum porrectum ct.citral as materials.The results show that the rooting rate of micro-cuttage of C.porrectum ct.citral increased with the longer acclimatization time.When micro-cuttage was performed after 11 days of acclimatization,the rooting rate reached 90.91%.The rooting rate of micro-cuttage of C.porrectum ct.citral increased with the increase of IBA concentration.The highest rooting rate(83.33%),greatest number of root number,the largest root length and thickness were observed when the concentration of IBA reaches 4 000 mg/L.Additionally,adventitious roots appeared at the base of the tissue culture seedlings 14 days after micrio-cuttage,showing a faster rooting speed and improved rooting rate compared to conventional cuttings.The mixture medium of vermiculite and peat significantly improved the rooting of the micro-cuttage of C.porrectum ct.citral,with a 2:1 ratio of vermiculite to peat being the optimal substrate,resulting in the highest rooting rate of 86.25%and the greatest number of roots.The study identifies the optimum conditions for micro-cutting of C.porrectum ct.citral tissue plantlets were obtained,which could provide reference for the well-bred breeding of C.porrectum.
万方数据
维普
