Analysis of WRKY transcription factors in Cinnamomum camphora
The transcription factor WRKY gene family plays a significant role in hormone regulatory pathways,plant growth and development,and the up-and-down regulation of other genes'expression.In order to study the function of the WRKY gene family of Cinnamomum camphora and better understand its regulatory mechanism for terpenoids,the WRKY transcription factors of C.camphora were identified and analyzed by bioinformatics methods based on the obtained whole genome and transcriptome data of different chemotypes of C.camphora.The results showed that the expression regulation of the transcript levels of key TPS genes might be influenced by C.camphora WRKY transcription factors.Using HMMER 3.0 software,61 potential CcWRKY sequences were identified by searching and comparing the C.camphora protein sequence data for WRKY genes.Group Ⅰ,Group Ⅱ,and Group Ⅲ were the initial classifications for WRKY proteins based on the number of conserved domains and zinc finger structure.The Group Ⅱ WRKY genes could be further divided into five subgroups based on their structural characteristics:Ⅱa,Ⅱb,Ⅱc,Ⅱd,and Ⅱe.Analysis of the WRKY conserved domains of CcWRKY proteins revealed structural mutations in the three WRKYGQK heptapeptide series on Group Ⅱ proteins.The analytical results revealed that there are seven different varieties of C.camphora WRKY gene structures and expression patterns:those with no intron and those with one,two,three,four,five,and six introns.All CcWRKY genes were expressed on the transcriptional data,and among them,CCA009569.1,CCA002757.1,and CCA027442.1 were expressed much higher in Linalool-type C.camphora than the other four chemotypes,and it can be hypothesized that they may be involved in the regulatory pathway of Linalool synthesis.