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樟树WRKY转录因子分析

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WRKY基因家族是一种重要的转录因子,参与植物生长发育及激素调节通路,可以正向或负向调节其他基因的表达.为了研究樟树WRKY基因家族的功能,更好地了解其对萜类物质的调控机制,研究基于已获得的樟树全基因组及不同化学型转录组数据,采用生物信息学方法对樟树WRKY转录因子进行鉴定与分析.结果表明:TPS关键基因转录水平的表达调控可能受樟树WRKY转录因子的影响.使用HMMER 3.0软件对樟树蛋白序列进行WRKY基因的检索比对,分离出61条候选CcWRKY序列.根据WRKY保守域数目和锌指结构类型,WRKY蛋白被分为Group Ⅰ、Group Ⅱ、Group Ⅲ3种类型,其中Group Ⅱ型WRKY基因依据其结构特征可进一步分为5个亚组:Ⅱa、Ⅱb、Ⅱc、Ⅱd和Ⅱe.对CcWRKY蛋白进行WRKY保守域分析,发现Ⅱ型蛋白上的3条WRKYGQK七肽系列结构上发生了突变.对CcWRKY基因的结构和表达模式进行分析,结果表明其结构可以分为不含内含子、含有1~6个内含子7种.所有CcWRKY基因在5种化学类型转录谱中均有表达,其中CCA009569.1、CCA002757.1、CCA027442.1在芳樟中的表达量远高于其他4种化学型,推测其可能参与芳樟醇的合成调控途径.
Analysis of WRKY transcription factors in Cinnamomum camphora
The transcription factor WRKY gene family plays a significant role in hormone regulatory pathways,plant growth and development,and the up-and-down regulation of other genes'expression.In order to study the function of the WRKY gene family of Cinnamomum camphora and better understand its regulatory mechanism for terpenoids,the WRKY transcription factors of C.camphora were identified and analyzed by bioinformatics methods based on the obtained whole genome and transcriptome data of different chemotypes of C.camphora.The results showed that the expression regulation of the transcript levels of key TPS genes might be influenced by C.camphora WRKY transcription factors.Using HMMER 3.0 software,61 potential CcWRKY sequences were identified by searching and comparing the C.camphora protein sequence data for WRKY genes.Group Ⅰ,Group Ⅱ,and Group Ⅲ were the initial classifications for WRKY proteins based on the number of conserved domains and zinc finger structure.The Group Ⅱ WRKY genes could be further divided into five subgroups based on their structural characteristics:Ⅱa,Ⅱb,Ⅱc,Ⅱd,and Ⅱe.Analysis of the WRKY conserved domains of CcWRKY proteins revealed structural mutations in the three WRKYGQK heptapeptide series on Group Ⅱ proteins.The analytical results revealed that there are seven different varieties of C.camphora WRKY gene structures and expression patterns:those with no intron and those with one,two,three,four,five,and six introns.All CcWRKY genes were expressed on the transcriptional data,and among them,CCA009569.1,CCA002757.1,and CCA027442.1 were expressed much higher in Linalool-type C.camphora than the other four chemotypes,and it can be hypothesized that they may be involved in the regulatory pathway of Linalool synthesis.

Cinnamomum camphoraWRKY transcription factorsgene familyexpression pattern

伍艳芳、涂白连、张月婷、刘新亮、郑永杰

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江西省林业科学院,国家林业草原樟树工程技术研究中心,江西 南昌 330013

樟树 WRKY转录因子 基因家族 表达模式

2024

南方林业科学
江西省林业科学院,江西省林学会

南方林业科学

影响因子:0.56
ISSN:1006-2505
年,卷(期):2024.52(5)