Study on tissue-cultured and rapid propagation technology of ancient trees of Glyptostrobus pensilis
Glyptostrobus pensilis is a famous critically endangered relict plant in China,and most of the extant wild individuals are ancient trees.In order to further conserve the precious wild genetic resources of G.pensilis,we used the shoot segments of extant G.pensilis in Jiangxi Province as the explant material,and investigated the effects of different basic medium,and plant growth regulators on the bud induction and proliferative cultivation stages of G.pensilis.The research results indicated that the optimal shoot induction and proliferation culture medium for the G.pensilis was MS+30 g·L-1 sucrose+5 mg·L-1 Vc+0.1 mg·L-1 zeatin+7 g·L-1 agar.Among the selected materials,the effect of Yiyang 13(yy13)plantlets induction and proliferation was the best among the ancient trees of G.pensilis,which was suitable as an explant for excellent proliferation and subculture.
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