Effects of different shading treatments on the growth and physiological characteristics of Pseudostellariae heterophylla at seedling stage
In order to explore the effect of shading on the growth and physiological characteristics of Pseudostellaria heterophylla,30%and 50%shading and full light treatments were applied to determine the effects of different shading treatments on the growth and some physiological indexes of P.heterophylla.The results showed that with the increase of shading intensity,the growth indexes such as leaves number,branch number,root number,root length and biomass of P.heterophylla increased first and then decreased,and the growth of P.heterophylla was the best under 30%shading treatment.The contents of superoxide dismutase,peroxidase,catalase,ascorbate peroxidase and malondialdehyde in leaves were 129.74 U∙g-1,789.32 U∙(g∙min)-1,34.82 U∙(g∙min)-1,211.77 U∙(g∙min)-1FW and 0.06 µmol∙g-1,compared with the control,they decreased by 35.52%,31.04%,13.73%,23.77%and 45.45%,respectively.With the increase of shading intensity,both the soluble sugar content(69.53 mg∙g-1)and soluble protein content(4.27 mg∙g-1)of the leaves treated were the highest.The difference in chlorophyll a and chlorophyll b content between the two shading treatments was not significant(P>0.05),but significantly higher than that of the full light treatment(P<0.05).The carotenoid content in leaves treated with 30%shading was 0.12 mg·g-1,significantly higher than that in full light treatment(P<0.05).So,in the process of artificial cultivation,the shading degree of about 30%was the most beneficial to the growth and physiology of the seedling stage of P.heterophylla.