Establishment and optimization of the regeneration system of Dalbergia odorifera'Hailin No.1'
To establish a tissue culture system for the stem segments of Dalbergia odorifera,the newly sprouted stem segments of D.odorifera'Hailin No.1'were used as explants to study the effects of different sampling times,basic culture media,disinfection schemes,exogenous hormones,and antibiotic additions on stem segment induction,adventitious shoot proliferation,and bottle seedling rooting.The results showed that,the best time for explants to be taken was from late April to mid May,and the best disinfection method was 1/15 ClO2 for 7 min,and 1/2 MS as basic medium.The hormone formula for the first generation induction of stem segments in the basic culture medium was 6-BA 1.50 mg·L-1+NAA 0.20 mg·L-1.When chloramphenicol(100.00 mg·L-1)and rifampicin(100.00 mg·L-1)were added into the medium,the rate of axillary bud induction was 84.24%,and the contamination rate was only 13.89%.The optimal multiplication formula was 1/2 MS+6-BA 2.0 mg·L-1+NAA 0.10 mg·L-1 and the multiplication coefficient was 3.33.The best rooting medium was 1/2 MS+6-BA 0.15 mg·L-1+NAA 2.50 mg·L-1,the rooting rate was 78.33%.
Dalbergia odorifera'Hailin No.1'stem segmenttissue cultureregeneration system
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