In order to explore the quality difference of Gardenia jasminoides in different regions,142 batches of G.jasminoides from different origin in Jiangxi Province were selected.The content of gardenoside and crocin Ⅰ was determined by high performance liquid chromatography,and the composition levels of gardenia from different regions were compared.The results showed that,(1)The average content of gardenoside and crocin Ⅰ in G.jasminoides from different regions of Jiangxi Province was 3.94%and 0.91%,with G.jasminoides harvested in Fengcheng City having an average content of 5.15%and 0.82%for gardenoside and crocin Ⅰ,Gongqingcheng City having an average content of 3.29%and 1.01%for gardenoside and crocin Ⅰ,and Zhangshu City having an average content of 3.28%and 0.89%for gardenoside and crocin Ⅰ.(2)There were significant differences in the content of gardenoside and crocin Ⅰ in G.jasminoides from different regions,and the regional characteristics were obvious.The average content of gardenoside in gardenia harvested in Fengcheng City was the highest,at 5.15%.The content of geniposide in GY,HC,WN,XGXH and YF gardenia clones collected in Fengcheng City was significantly higher than that in Gongqingcheng City and Zhangshu City collection areas(P<0.05).The average content of crocin Ⅰ in G.jasminoides harvested in Gongqingcheng City was the highest,at 0.98%.The content of crocin Ⅰ in GY,HC,WN,XGXH and YF gardenia clones collected in Fengcheng City was significantly higher than that in Gongqingcheng City and Zhangshu City collection areas(P<0.05).The quality of 142 batches of G.jasminoides from different regions of Jiangxi Province meets the requirements of the pharmacopoeia.