Effects of Low Temperature Stress on Active Oxygen Metabolism of Walnut Branches
Two walnut cultivars “Luguo No .12” ( most cold-resistant cultivar ) and“Xiangling” ( more cold-resistant culti-var) were taken as test materials , they were stressed and treated at -15, -20, -25, -30 and -35 ℃, the plasma membrane relative permeability, contents of MDA and H2O2, producing rate of O2 -· , and activities of protective enzymes (SOD and CAT) in their isolated braches were determined , and the effects of low temperature stress on the active oxygen metabolism of walnut bran -ches were analyzed .The results indicated that the plasma membrane permeability , producing rate of O 2 -· , and contents of MDA and H2 O2 in the branches of two walnut cultivars were significantly increased by low temperature stress , but their increment in “Lu-guo No.12” was lower than that in “Xiangling”.The SOD activity in two cultivars all presented the trend of “ascend -descend-ascend -descend”, while the CAT activity increased firstly and then decreased under low temperature stress .It was concluded that “Luguo No.12” could effectively enhance the activities of protective enzymes , further inhibit the accumulation of H 2 O2 during low temperature stress, therefore reduce the damage of active oxygen to walnut .This might be one of important reasons for higher cold-resistance of “Luguo No.12”.
WalnutLow temperature stressActive oxygen metabolism