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低温胁迫对核桃枝条活性氧代谢的影响

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以抗寒性不同的香玲和鲁果12号核桃品种为试材,在-15、-20、-25、-30、-35℃低温梯度胁迫下,分别测定了离体枝条的质膜相对透性、MDA和H2 O2含量、O2-·产生速率、SOD和CAT保护酶活性等指标,分析了低温胁迫对核桃枝条活性氧代谢的影响,结果表明:低温胁迫显著增加了核桃枝条的膜透性、O2-·产生速率、MDA和H2 O2含量,但耐寒品种鲁果12号的增幅低于香玲品种;2个品种的SOD酶活性均呈现“升-降-升-降”的趋势,CAT酶活性则先升高后降低,但不同品种之间的变化幅度较大;由此可知,鲁果12号在低温胁迫期间可有效地增加保护酶活性,抑制H2 O2的积累,从而降低活性氧对核桃的伤害,这可能是其抗寒性较强的重要原因之一。
Effects of Low Temperature Stress on Active Oxygen Metabolism of Walnut Branches
Two walnut cultivars “Luguo No .12” ( most cold-resistant cultivar ) and“Xiangling” ( more cold-resistant culti-var) were taken as test materials , they were stressed and treated at -15, -20, -25, -30 and -35 ℃, the plasma membrane relative permeability, contents of MDA and H2O2, producing rate of O2 -· , and activities of protective enzymes (SOD and CAT) in their isolated braches were determined , and the effects of low temperature stress on the active oxygen metabolism of walnut bran -ches were analyzed .The results indicated that the plasma membrane permeability , producing rate of O 2 -· , and contents of MDA and H2 O2 in the branches of two walnut cultivars were significantly increased by low temperature stress , but their increment in “Lu-guo No.12” was lower than that in “Xiangling”.The SOD activity in two cultivars all presented the trend of “ascend -descend-ascend -descend”, while the CAT activity increased firstly and then decreased under low temperature stress .It was concluded that “Luguo No.12” could effectively enhance the activities of protective enzymes , further inhibit the accumulation of H 2 O2 during low temperature stress, therefore reduce the damage of active oxygen to walnut .This might be one of important reasons for higher cold-resistance of “Luguo No.12”.

WalnutLow temperature stressActive oxygen metabolism

相昆、徐颖、王新亮、李国田、张美勇

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山东省果树研究所,山东 泰安271000

核桃 低温胁迫 活性氧代谢

2014

江西农业学报
江西省农业科学院 江西省农学会

江西农业学报

CSTPCD
影响因子:0.632
ISSN:1001-8581
年,卷(期):2014.(1)
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