Study on Stem-tip Tissue Culture and Rapid Propagation Techniques of Strawberry “Ningyu”
The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS +0.5 mg/L 6-BA+0.1 mg/L NAA;the best medium for subcultural propagation was MS +0.5 mg/L 6-BA+0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS+0.05 mg/L NAA, and the rooting rate reached 96.6%.
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NSTL
万方数据
维普
