Control Effect of Two Strains of Biocontrol Bacteria Mixed with Bacillus thuringiensis on Thosea sinensis in Tea Garden
To select a biocontrol bacterial mixture formula with high control effect on Thosea sinensis Walker to replace chemical pesticides,the study used a method of pairwise mixing of biocontrol bacteria.Two strains of biocontrol bacteria(X-2,X-11)selected from the gut of the naturally dead Thosea sinensis Walker found in tea gardens were mixed with Bacillus thuringiensis(Bt).Indoor toxicity tests showed that the X-2 and X-11 biocontrol bacteria had a synergistic effect with Bt,which can improve the control effect on the Thosea sinensis Walker.Especially,the mixed microbial agent made by mixing 1×108/mL of X-11 bacterial solution and 32 IU/mg of Bt had the best control effect on Thosea sinensis Walker,with a maximum mortality rate of 81.50%after 72 hours,which was 22.9 percent points higher than that of the X-11.Field experiments showed that after spraying the mixed microbial agents selected in tea gardens for 10 days,the highest control effect on the Thosea sinensis Walker could reach 67.20%,which was 8.8 percent points higher than that of the Bt.This study can provide a theoretical basis for the development and utilization of biological control agents against Thosea sinensis Walker in the future.
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